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KMID : 0603820150210010023
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Journal of Experimental & Biomedical Science
2015 Volume.21 No. 1 p.23 ~ p.31
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The Characterization of Anti-HER-2/neu Monoclonal Antibody using Different in vivo Imaging Techniques
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Moon Cheol
Kim Eun-Jung
Choi Dan-Bee
Kim Byoung-Soo
Kim Sa-Hyun
Choi Tae-Hyun
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Abstract
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Recently, specific antibodies have been used extensively to diagnose and treat various diseases. It is essential to assess the efficacy and specificity of antibodies, especially the in vivo environment. Anti-HER-2/neu mAb was evaluated as a possible transporting agent for radioimmunotherapy. The monoclonal antibody was successfully radio-labeled with 131I. In vitro binding assays were performed to confirm its targeting ability using another radio-iodine, 125I. Binding percentage of 125I labeled anti-HER-2/neu mAb in HER-2/neu expressing CT-26 cells was found to be 4.5%, whereas the binding percentage of 125I labeled anti-HER-2/neu mAb in wild-type CT-26 was only 0.45%. In vivo images were obtained and analyzed through γ-camera and an optical fluorescent modality, IVIS-200. γ-camera images showed that 131I labeled anti-HER-2/neu mAb accumulated in HER-2/neu CT-26 tumors. Optical imaging based on near infrared fluorescence labeled anti-HER-2/neu mAb showed higher fluorescence intensities in HER-2/neu CT-26 tumors than in wild-type CT-26 tumors. Anti-HER-2/neu mAb was found to specifically bind to its receptor expressing tumor. Our study demonstrates that in vivo imaging technique is a useful method for the evaluation of an antibody"s therapeutic and diagnostic potentials.
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KEYWORD
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Radioimmunotherapy (RIT), In vivo imaging, Monoclonal antibody, HER-2/neu oncogene
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